| APLN

Apelin (also known as APLN) is a peptide encoded by the APLN gene. Apelin is one of two endogenous ligands for the G-protein-coupled APJ receptor that is expressed at the surface of some cell types. It is widely expressed in various organs such as the heart, lung, kidney, liver, and human plasma.The sites of receptor expression are linked to the different functions played by apelin in the organism. The apelin receptor is expressed early during the embryonic development of the heart, where it regulates the migration of cell progenitors fated to differentiate into cardiomyocytes, the contractile cells of the heart.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	8000	4000	2000	1000	500	250	125	0
OD	2.3	1.87	1.25	0.82	0.55	0.41	0.32	0.09
Corrected OD	2.21	1.78	1.16	0.73	0.46	0.32	0.23	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human APLN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-103	98
Plasma(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Human APLN and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-96	88-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98